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Timothy Johnson

Timothy JohnsonCooperating Doctoral Program: Plant, Soil and Microbial Sciences

EITS Track: Environmental Toxicology

Education: B.S., Environmental Soil Science, Brigham Young University

Research Interests:
I am studying bacterial Rieske Dioxygenase genes with aerobic degrading capacity toward (Cl)-dibenzo-p-dioxin. There are relatively few of these genes known. We are trying to describe the diversity of these genes in the environment through PCR based pyrosequencing , culturing, and isolation techniques. In order to complete the PCR based pyrosequencing, new primers were designed and are now being tested with environmental samples. This will be the topic of my dissertation.

I am also completing research in a second area of Antibiotic Resistance Genes. We are attempting to understand the diversity and abundance of antibiotic resistance genes in the environment and determine the role horizontal gene transfer may play in their transfer. We are developing a comprehensive microarray to probe for all known antibiotic resistance genes from antibiotic resistance strains isiolated from environmental samples.

Major Professor: James Tiedje, Crop and Soil Sciences

Contact: john2929@msu.edu